Richard Shand
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Richard F. Shand, PhD | ||||
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Professor of Microbiology Department of Biological Sciences South Beaver at Franklin Northern Arizona University Flagstaff, AZ 86011-5640 |
Office: Room 239, Building 88 Lab: Room 229, Building 88 (928) 523-9970 (office) (928) 523-6145 (lab) (928) 523-7500 (Fax) |
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E-mail: Richard.Shand@nau.edu |
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Molecular Biology, Biochemistry, Physiology and Genetics of Extremely Halophilic Archaea (The Haloarchaea)
Research
Interests Introduction:
The domain Archaea
occupies an intermediate phylogenetic position between the domains Bacteria
and Eucarya, and is
characterized by three main biotypes:
hyperthermophiles, methanogens and extreme halophiles (unrooted
phylogenetic tree).
The extremely halophilic Archaea
(the haloarchaea) require external concentrations of 2 to 4.5 molar NaCl
for optimal growth.
As a group, they have capitulated evolutionarily to these high salt
conditions by maintaining an even higher internal salt concentration,
which in the most extreme halophiles is about 5 molar (3 M K+,
1 M Na+ and 1 M Mg++).
Consequently, haloarchaeal proteins and other macromolecules have
evolved to operate in this salty milieu, with many proteins requiring high
salt conditions for activity.
In addition, the haloarchaea can be slightly thermophilic (e.g., Haloferax
mediterranei grows optimally between 47 and 54°C) and can grow over a
broad range (>50°C) of temperatures (Arrhenius
plot for Haloferax mediterranei).
The
Archaea possess a minimalist version of the eucaryotic Pol II basal
transcription apparatus: in
addition to a TATA box element, factors analogous to the eucaryal system
include a multisubunit Pol II-like polymerase, TATA binding proteins (TBPs)
and transcription factor B (TFB) proteins.
Focus 1: Regulation of Gene Expression.
My research has focussed on regulation of gene expression, first in
Salmonella typhimurium
(regulation of the histidine biosynthetic operon by the “alarmone”
ppGpp) and then in the haloarchaea (regulation of the bacterio-opsin gene
cluster in Halobacterium salinarum).
Currently, my main focus is stationary phase gene expression in the
haloarchaea, using halocin genes as models.
Halocin genes are induced as the cells transition between
exponential phase and stationary phase.
We are in the process of identifying trans-acting
regulatory factors that control their expression. Focus 2: Isolation and Characterization of Halocins. Production
of protein antibiotics is a nearly universal feature of all living
organisms: bacteria produce bacteriocins (e.g., colicins, microcins and
lantibiotics); and eukaryotes including protozoans, insects, horseshoe
crabs, frogs, mammals (including humans) and plants, produce a wide
variety of antimicrobial peptides. A
second area of research involves the isolation and characterization of
haloarchaeal antimicrobials called "halocins":
protein antibiotics that are externalized by the producer cells and
kill or inhibit other organisms (halocin
assay picture). Some
halocins are small peptides (e.g., the microhalocins S8 and R1; <5 kDa)
while others are ten times as large (e.g., halocin H4).
Projects in this area include determination of their 3D structure,
heterologous expression, mechanism of action, immunity mechanism, and
spectrum of activity (which can be very broad) (halocin
inhibition of Sulfolobus picture).
Focus 3: Recovery of Haloarchaea from the Environment. Surveys of both planktonic hypersaline environments and salt rocks utilizing amplification of 16S rDNA by PCR have shown that the diversity of organisms in these environments is far greater than what can be recovered and grown. A third area of research involves the development of new methods for improved recovery of haloarchaea and other extreme halophiles from planktonic hypersaline environments, from surface salt deposits and salt rocks, and from halite (recovery protocol coming soon). |
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Dick Shand, Arizona |
Liz O'Connor, Maryland |
Last updated: 02/16/2006